@article{Maimoona Khan and Shahdab Ahmed Butt_2016, title={Effect of Caffeine and Vitamin D3 on Body Weight of Balb/C Mice}, volume={20}, url={https://journalrmc.com/index.php/JRMC/article/view/160}, abstractNote={<p>Background: To evaluate effect of caffeine and<br>vitamin D3 on body weight of BALB/c mice.<br>Methods: In this experimental study thirty BALB/c<br>mice, three weeks old with average weight 12-14 g<br>were taken and divided into three groups with 10<br>mice (5 male, 5 female) in each group. The control<br>group G1 was given normal diet prepared at National<br>Institute of Health Islamabad with liberal water<br>intake. Each animal in experimental group G2 was<br>given 10mg of caffeine per100g body weight on<br>alternate day, three days in a week by oral gavage for<br>60 days. All animals of G3 were given caffeine<br>10mg/100g body weight on alternate day, three days<br>a week and vitamin D3 0.1μg per day by oral gavage<br>for 60 days. The effect of caffeine and vitamin D3<br>was assessed by measuring the body weight of the<br>mice at the end of the experiment.<br>Results: Mean final body weight of mice of control<br>group G1 was 31.2 ± 1.932 g while the mean final<br>body weight of experimental groups G2 and G3 was<br>found to be 27.1 ± 2.025 g and 29.2 ± 2.098 g . Mean<br>final body weight of mice of experimental group G2<br>was less as compared to control group G1 and<br>experimental group G3.<br>Conclusion: The body weight of the mice was<br>reduced due to caffeine consumption while vitamin<br>D3 administration ameliorated the deleterious effect<br>of caffeine on the body weight.</p>}, number={4}, journal={Journal of Rawalpindi Medical College}, author={Maimoona Khan and Shahdab Ahmed Butt}, year={2016}, month={Dec.} }