TY - JOUR AU - Moizza Sahar, Fauzia Shafi Khan, Yusra Rashid, Farhana Shehzad, Ghulam Mustafa, Shahida Mohsin, Moizza Sahar, Fauzia Shafi Khan, Yusra Rashid, Farhana Shehzad, Ghulam Mustafa, Shahida Mohsin PY - 2019/05/02 Y2 - 2024/03/28 TI - Significance of Neuropilin-1 (CD 304) Expression in Paediatric B- Lineage Acute Lymphoblastic Leukemia (ALL) JF - Journal of Rawalpindi Medical College JA - JRMC VL - 23 IS - 1 SE - Articles DO - UR - https://journalrmc.com/index.php/JRMC/article/view/1075 SP - AB - <p>Background: To determine the prognostic <br>significance of NRP-1 (CD304) expression in <br>paediatric B-lineage Acute Lymphoblastic <br>Leukaemia (ALL) patients. <br>Methods: In this comparative study newly <br>diagnosed cases (aged 1-15 years) of B-ALL were <br>selected. Age and sex matched, 21 healthy controls <br>were also included in the study to assess the NRP-1 <br>expression on peripheral blood lymphocytes. A <br>minimum 1ml of blood and bone marrow aspirate <br>samples were taken in EDTA vacutainer and <br>immunophenotyping was done on gated blast cells <br>using an extensive panel of antibodies including <br>myeloid markers (CD13, CD33 and cytoplasmic anti-<br>MPO) and lymphoid markers (CD34, CD2, CD3, <br>CD5, CD7, CD4, CD8, HLA DR, CD10, CD19, CD22; <br>cytoplasmic CD3, CD22, CD79a and nuclear anti-<br>TdT). The sample was considered NRP-1 positive if <br>20% or more of the gated blast cells expressed it . <br>Man-Witney U test and Kruskal Wallis test were <br>used for non-parametric data. The p value &lt;0.05 was <br>considered significant. <br>Results: Out of 66 B-ALL patients, 53% were males. <br>There were 20 (30%) NRP-1 positive and 46 (70%) <br>NRP-1 negative patients. The prognosis of NRP-1 <br>positive group was poor as compared to NRP-1 <br>negative group with high blast percentage (80%) (p= <br>0.042), low morphological remission rate (21%) <br>(p=0.004) and low survival rate (29%) (p=0.009). The <br>mean survival days in dead patients was also less <br>(22.75 days). <br>Conclusion: NRP-1 over expression is associated <br>with disease progression and severity in paediatric <br>B-ALL patients.</p> ER -