Effect of Caffeine and Vitamin D3 on Body Weight of Balb/C Mice

Abstract

Background: To evaluate effect of caffeine and
vitamin D3 on body weight of BALB/c mice.
Methods: In this experimental study thirty BALB/c
mice, three weeks old with average weight 12-14 g
were taken and divided into three groups with 10
mice (5 male, 5 female) in each group. The control
group G1 was given normal diet prepared at National
Institute of Health Islamabad with liberal water
intake. Each animal in experimental group G2 was
given 10mg of caffeine per100g body weight on
alternate day, three days in a week by oral gavage for
60 days. All animals of G3 were given caffeine
10mg/100g body weight on alternate day, three days
a week and vitamin D3 0.1μg per day by oral gavage
for 60 days. The effect of caffeine and vitamin D3
was assessed by measuring the body weight of the
mice at the end of the experiment.
Results: Mean final body weight of mice of control
group G1 was 31.2 ± 1.932 g while the mean final
body weight of experimental groups G2 and G3 was
found to be 27.1 ± 2.025 g and 29.2 ± 2.098 g . Mean
final body weight of mice of experimental group G2
was less as compared to control group G1 and
experimental group G3.
Conclusion: The body weight of the mice was
reduced due to caffeine consumption while vitamin
D3 administration ameliorated the deleterious effect
of caffeine on the body weight.