Official publication of Rawalpindi Medical University
Ameliorative Effect of Zinc on Bone Cells of Humerus and Femur of Female Rats Under High Salt Diet
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Kaukab Anjum , Hafsa Nisar , SumairaAbbasi. Ameliorative Effect of Zinc on Bone Cells of Humerus and Femur of Female Rats Under High Salt Diet. JRMC [Internet]. 2016 Sep. 30 [cited 2024 Mar. 29];20(3). Available from: http://journalrmc.com/index.php/JRMC/article/view/184

Abstract

Background:To evaluate the effect of zinc on bone
cells of humerus and femur of female adult Sprague
Dawley rats under high salt diet.
Methods: In this experimental study 10-12 weeks
old,45 adult female Sprague Dawley rats were used.
Three groups were made, each having fifteen rats.
Control group C received laboratory diet without
any alteration. Experimental group A was served
with high salt diet (8%NaCl)whereas experimental
group B animals were given high salt diet
augmented with zinc (50mg/kg/day).All groups were
given the diet for eight weeks after which they were
sacrificed and left humeri and femora of all rats were
obtained.After decalcification, Bone tissue from
proximal part of shaft was attained to study the
number of osteoblast (bone forming) and osteoclast
(bone resorbing) cells.For light microscopy, tissue
processing was done to obtain five micrometer (μm)
sections. Tissues were stained with Haematoxylin
and eosin (H&E).The results were compiled and
compared.
Results: After salt administration, group Animals
showed marked increase in osteoclast number
whereas the osteoblast number exhibited substantial
decrease. Protective effects were seen in zinc
supplemented experimental group B with decrease
in osteoclast number and increase in osteoblast
number.
Conclusion:Zinc has protective effect against high
salt induced damage in the bone cells of rats

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