Urinary Tract Bacterial Pathogens and their Sensitivity Pattern

Abstract

Background:To detect the prevalence rate of bacterial infection among urinary isolates and to determine the antimicrobial susceptibility pattern.
Methods In this descriptive study 300 urine samples were collected for culture and sensitivity. After documenting pus cells, inoculation was done on required medias. Growth so obtained was interpreted as one organism grows into one colony hence called colony forming unit (CFU). The number of colonies formed were counted representing number of organisms present in the inoculum of urine taken and a total count was calculated for I ml of urine. Growth of bacterial count ≥ 105 CFU/ml was considered significant. The significant growth obtained was processed for standard biochemical tests of identification and in vitro sensitivity pattern(by the standard disc diffusion method). Zones of inhibition were measured and interpreted by the recommendations of clinical and laboratory standards.
Results:- Total 250 (50%) samples of both males and females were positive. Females showed higher prevalence rate of UTI than males. Gram negative bacteria were found in high prevalence rate than gram positive bacteria. The common organisms were Escherichia coli, Klebsiella pneumonae, Staphylococus saprophyticus, Proteus mirabilis, Staphylococus aureus and Pseudomonas aeroginosa.. In vitro antibiotic susceptibility tests reveal that gram negatives bacteria were sensitive to imipenum, nitrofurantoin and norfloxacin. Gram positive isolates were sensitive to nitrofurantoin, amoxicillin / clauvanic acid, and ampicillin.
Conclusion:-There is a need for constant monitoring of susceptibility of specific pathogens in different populations to commonly used anti-microbial agents.