Department of Pulmonology, Pakistan Institute of Medical Sciences, Islamabad

  • Naureen Saeed Benazir Bhutto Hospital and Rawalpindi Medical College
Keywords: : DENV, Dengue virus, Dengue Shock Syndrome, Dengue Haemorrhagic Fever,


Background: To determine the association of ELISA based serological markersNS1 antigen, IgM, IgG antibodies and RT-PCR in the diagnosis of dengue virus infection

Methods: In this descriptive cross sectional study 420 serum samples from patients with suspicion of dengue fever were tested for detection of dengue by NS1 antigen ELISA, IgG, IgM ELISA. RT-PCR for dengue was carried out in all NS1 antigen ELISA positive cases for confirmation of dengue.

Results: Out of 420 cases , 249 cases were positive for either one of the three markers NS1, IgM,IgG. Males constituted 71.66%.Two hundred and two (48.09%) were positive for NS1 only,13 (3.09%) were positive for NS1 and IgG, 07 (1.66%) were NS1, IgM and IgG positive,16 (3.80%) were positive for IgG only ,11 (2.61%) were positive for NS1 and IgM whereas 171 (40.17%) samples were reported negative for NS1, IgM and IgG.RT-PCR was conducted on 233 NS1 positive cases out of which 80.06% cases turned out positive. Maximum number of cases belonged to DEN-2 genotype.

Conclusion: Early diagnosis helps in improved patient care, suitable treatment, prevents severe complications and helps limit the spread of the disease. RT PCR is  a reliable test for the  diagnosis of acute dengue fever.

How to Cite
Saeed, N. (2017). Department of Pulmonology, Pakistan Institute of Medical Sciences, Islamabad. Journal of Rawalpindi Medical College, 21(4), 335-338. Retrieved from