Antiulcerogenic Activity of Vitamin E on Gastric Ulcers produced by Indomethacin

  • Ayesha Yousaf
  • Ifra Saeed
  • Tehzeeb ul Hassan
  • Rana Fahd Intisar
  • Mohammad Amin Shiekh
Keywords: Gastric Ulcers, Indomethacin, Vitamin E.

Abstract

Objective: To study the morphological and histological effects of vitamin E on gastric lesions produced by indomethacin.
Materials and Methods: This was an animal interventional study, 48 adult healthy albino mice were selected and were split into four groups A, B, C and D. Number of animals in each group was 12. Group A was categorized as control. Evion 400mg/kg was administered to Group B. Indocid 25mg/kg was given to group C. Indocid and Evion both (25mg/kg &400 mg/kg respectively) were administered to Group D. In all group, six animals were selected and treated for three days and rest for eight days with calculated doses of drugs. Mice were sacrificed and dissection was done 24 hours after the last dose. The stomach was identified, washed, and observed under dissecting microscope to study the number and shape of ulcers. The dimension of ulcers was measured under a compound microscope.
Results: No ulcers were seen in groups A and B. 35 and 10 ulcers were observed in groups C and D respectively. The mean number of ulcers in groups C and D was statistically significant (p-value=0.000). In comparison to group D, Pindot, linear, Irregular, and punched-out ulcers were more prevalent in group C and were statistically significant (p-value < 0.05). The mean linear dimension of ulcers in group C was much greater than in group D. The mean dimension of ulcers in group C1 and C2 was 262.50µm and 232.5µm respectively. Whereas in group D1 and D2 given both Indocid and vitamin E the dimensions were 56.5µm and 50µm.
Conclusion: Vitamin E has an anti-ulcerogenic effect on stomach mucosa by reducing the number and dimension of ulcers.

References

1. Volker boham. Vitamin E Antioxidants, Basel; 2018: Mar 7(3): 44.
2. Zingg J.-M. Vitamin E: An overview of major research directions Mol. Asp. Med; 2007:28: 400–422.
3. Omar A Masri 1, Jean M Chalhoub 1, Ala I Sharara Role of vitamin E in gastrointestinal disease World J Gastroenterol;2015: 7;21(17):5191-209
4. Reboul E. Vitamin E bioavailability: Mechanisms of intestinal absorption in the spotlight. Antioxidants; 2017:6: 95.
5. Sugimoto N, Yoshida N, Nakamura Y, Ichikawa H, Naito Y, Okanoue T, et al. Influence of vitamin E on gastric mucosal injury induced by Helicobacter pylori infection. Biofactors. 2006 Jan 1;28(1):9-19.
6. Ilahi M, Khan J, Inayat Q, Abidi TS. Histological changes in parts of foregut of rat after administration. J Ayub Med coll Abottabad; 2006: 18(3):29
7. Ferrer-Pérez C, Martinez TE, Montagud-Romero S, Ballestín R, Reguilón MD, Miñarro J, et al. Indomethacin blocks the increased conditioned rewarding effects of cocaine induced by repeated social defeat. PLoS One. 2018 Dec 17;13(12):e0209291.
8. Chin KY, Ima-Nirwana S. The role of vitamin E in preventing and treating osteoarthritis–a review of the current evidence. Frontiers in pharmacology. 2018 Aug 21;9:946.
9. Kamisah Y, Qodriyah HMS, Chua KH, Azlina MFN. Vitamin E: A potential therapy for gastric mucosal injury.Pharmaceutical Biology; 2014:52(12):1591-1597
10. Asbaghi O, Sadeghian M, Nazarian B, Sarreshtedari M, Mozaffari-Khosravi H, Maleki V,et al. The effect of vitamin E supplementation on selected inflammatory biomarkers in adults: a systematic review and meta-analysis of randomized clinical trials. Scientific reports. 2020 Oct 14;10(1):1-7.
11. Taiwo VO, Conteh OL. The rodenticidal effect of indomethacin: pathogenesis and pathology.Veterinarski Arhvi; 2008: 78(2): 167-178.
12. Becker JC, Domschke W, Pohle T. Current approaches to prevent NSAID-induced gastropathy-COX selectivity and beyond. Br J Clin Pharmacol; 2004: 58(6): 587-600
13. Elahi M, Inayat Q, Wazir F, Huma z. Adaptation of rat gastric mucosa exposed to Indomethacin. A histological study. Gomal Journal of Medical Sciences; 2009: 7(2): 143-146.
14. Quinn PJ.Molecular associations of vitamin E. Vitamins and hormones; 2007: 76:67-98.
15. Mustafa C, Omer O, Olgun K, Hulya T, Mehmet Y.Protective effects of Vitamin E and Gingko biloba extract on stress induced gastric mucosal injury in rats. The Turkish Journal of Gastroenterology; 2001:12(1): 23-26.
Published
2021-03-30
How to Cite
1.
Yousaf A, Saeed I, Hassan T, Intisar R, Shiekh M. Antiulcerogenic Activity of Vitamin E on Gastric Ulcers produced by Indomethacin. JRMC [Internet]. 30Mar.2021 [cited 5Aug.2021];25(1):122-7. Available from: https://journalrmc.com/index.php/JRMC/article/view/1574