Abstract
Objective: This retrospective study's purpose was to provide insight into the distribution and trend of malaria cases in the Kohat District of KPK, Pakistan, from 2017 to 2021. The analysis of the sociodemographic traits, annual trends, species distribution, and seasonal fluctuations of malaria cases was the main goal of the study.
Methodology: The DHQ Divisional Headquarter Hospital in Kohat, which is overseen by the KPK, Peshawar malaria control program, provided the laboratory logbook from which the data was taken. Every suspected case of malaria that provided blood samples for microscopy was recorded in the study's logbook. We retrieved and analyzed data on the participant's demographics, pregnant status, diagnostic month and year, and parasite species found.
Results: 10,958 cases of malaria were verified out of 17,832 blood films that were analyzed during the study period. Males (53.2%) and those over the age of five (9,036 cases) accounted for the majority of cases. P. vivax accounted for 88% of the total species, with P. falciparum (10%) and mixed infections (2%), following in order. The majority of those affected were over the age of 15, and males were more likely than females to become infected. Cases of malaria were reported all year round, with summer and fall seeing the greatest transmission.
Conclusion: This study offers important new information about the five-year malaria trend in the Kohat District. The results emphasize the necessity of focused measures to alleviate the greater incidence of malaria in boys older than 15 years. The report also emphasizes how crucial monitoring and surveillance systems are to directing evidence-based treatments and maintaining advancements in the fight against malaria. Subsequent investigations ought to concentrate on executing specific measures and assessing their efficacy, and looking into the effects of climate change on regional transmission of malaria as well.
References
Gupta P. Rapid Diagnostic Tests for Malaria: Challenges and Future Prospects, a Brief Review. Challenges and Advances in Pharmaceutical Research Vol 8. 2022:152-62. DOI: 10.9734/bpi/capr/v8/3373C.
Shah H, Khan R, Naz F, Haseeb A, Jan A, Ullah R. Prevalence and distribution of malaria parasites in general population of district Dir Lower, Khyber Pakhtunkhwa, Pakistan. J Entomol Zool Studies. 2016;4(4):1211-15.
Khattak AA, Venkatesan M, Nadeem MF, Satti HS, Yaqoob A, Strauss K, et al. Prevalence and distribution of human Plasmodium infection in Pakistan. Malaria journal. 2013;12(1):1-8. Doi.org/10.1186/1475-2875-12-297.
Ullah R, Ayaz S, Zarin Sh AN. Prevalence of Plasmodium Vivax Using PCR Method in Afghan Refugee Ghamkol Camp District Kohat, Pakistan. Life Sci J. 2013;10(1):2948-53.
Zareen S, Zareen H, Zaman H, Rehman HU, Rehman NU, Nayab M, et al. Burden of Plasmodium vivax and Plasmodium falciparum in Afghan Refugee Camps located at Ghamkol Road Peshawar bypass District Kohat. 2016.
Khan SN, Ayaz S, Ali I, Attaullah S, Shams S, Zareen S, et al. Burden of Malaria infection among Neonates in highly epidemic region of Khyber Pakhtunkhwa, Pakistan. International Journal of Advancements in Research & Technology. 2013;2(4):84-92.
Jahan F, Khan NH, Wahid S, Ullah Z, Kausar A, Ali N. Malaria epidemiology and comparative reliability of diagnostic tools in Bannu; an endemic malaria focus in south of Khyber Pakhtunkhwa, Pakistan. Pathogens and Global health. 2019;113(2):75-85. Doi.org/10.1080/20477724.2019.1595904.
Khan AQ, Hussain S, Babar N, Samad A, Badshah N, Zaman S. Prevalent Occurrence of Plasmodium Falciparum Malaria in Pakistan. Pakistan Journal of Medical & Health Sciences. 2023;17(02):871. Doi.org/10.53350/pjmhs2023172871.
Miller LH, Ackerman HC, Su X-z, Wellems TE. Malaria biology and disease pathogenesis: insights for new treatments. Nature medicine. 2013;19(2):156-67. Doi.org/10.1038/nm.3073
Organization WH. High burden to high impact: a targeted malaria response. World Health Organization; 2018.
Khatoon L, Baliraine FN, Bonizzoni M, Malik SA, Yan G. Genetic structure of Plasmodium vivax and Plasmodium falciparum in the Bannu district of Pakistan. Malaria journal. 2010;9(1):1-10. Doi.org/10.1186/1475-2875-9-112.
Ndyomugyenyi R, Magnussen P, Lal S, Hansen K, Clarke SaE. Appropriate targeting of artemisinin‐based combination therapy by community health workers using malaria rapid diagnostic tests: findings from randomized trials in two contrasting areas of high and low malaria transmission in south‐western Uganda. Tropical medicine & international health. 2016;21(9):1157-70. Doi.org/10.1111/tmi.12748
Gaaib JN, Nassief AF, Al-Assi AJTJPS. Simple salting-out method for genomic DNA extraction from whole blood. 2011;16(2):1813-662.
ULLAH I. PREVALANCE OF MALARIA IN GENERAL POPULATION OF SHERGARH DISTRIC MARDAN.
Majid A, Rehman MU, Ahmad T, Ali A, Ali S, Ali S, et al. Prevalence of malaria in human population of district Mardan, Pakistan. World Journal of Zoology. 2016;11(1):63-6.
Akanbi F. Socio-economic and demographic impact on malaria prevalence in Akoko South-west of Ondo state, Nigeria. International Journal of Infectious Diseases. 2016;45:214. Doi.org/10.1016/j.ijid.2016.02.488.
Olusi T, Simon-Oke I, Akeju A. Composition, habitat preference and seasonal variation of malaria vector larval and pupa stage in Akure North Local Government Area of Ondo State, Nigeria. Bulletin of the National Research Centre. 2021;45:1-8. Doi.org/10.1186/s42269-021-00535-9.
Hussain I, Qureshi NA, Afzal M, Shaheen N, Ali A, Ashraf A. Prevalence and distribution of human Plasmodium infection in Federally Administrative Tribal Areas of Pakistan. Acta Parasitologica. 2016;61(3):537-43. Doi.org/10.1515/ap-2016-0071.
Baloch NI, Yasinzai MI, Shaheen G, Bibi B. 46. Endemicity of malaria in the human population of district Kech, Balochistan. Pure and Applied Biology (PAB). 2023;12(1):531-9. Doi.org/10.19045/bspab.2023.120055
Babure Z, Ahmed Y, Likasa S, Jiru F, Weldemarium T, Fite M. Trend analysis of malaria prevalence in East Wollega zone, oromia regional state, western Ethiopia, 2020: a retrospective study. J Women's Health Care. 2021;10(515):2167-420.
Kim J-R, Imwong M, Nandy A, Chotivanich K, Nontprasert A, Tonomsing N, et al. Genetic diversity of Plasmodium vivax in Kolkata, India. Malaria Journal. 2006;5(1):1-10. Doi.org/10.1186/1475-2875-5-71.
Manandhar S, Bhusal CL, Ghimire U, Singh SP, Karmacharya DB, Dixit SM. A study on relapse/re-infection rate of Plasmodium vivax malaria and identification of the predominant genotypes of P. vivax in two endemic districts of Nepal. Malaria Journal. 2013;12(1):1-8. Doi.org/10.1186/1475-2875-12-324.
Kattenberg JH, Gumal DL, Ome-Kaius M, Kiniboro B, Philip M, Jally S, et al. The epidemiology of Plasmodium falciparum and Plasmodium vivax in East Sepik Province, Papua New Guinea, pre-and post-implementation of national malaria control efforts. Malaria journal. 2020;19(1):1-18. Doi.org/10.1186/s12936-020-03265-x.
Okiring J, Epstein A, Namuganga JF, Kamya EV, Nabende I, Nassali M, et al. Gender difference in the incidence of malaria diagnosed at public health facilities in Uganda. Malaria Journal. 2022;21(1):1-12. Doi.org/10.1186/s12936-022-04046-4.
Gunda R, Chimbari MJ, Shamu S, Sartorius B, Mukaratirwa S. Malaria incidence trends and their association with climatic variables in rural Gwanda, Zimbabwe, 2005–2015. Malaria Journal. 2017;16(1):1-13. Doi.org/10.1186/s12936-017-2036-0.
Qureshi NA, Fatima H, Afzal M, Khattak AA, Nawaz MA. Occurrence and seasonal variation of human Plasmodium infection in Punjab Province, Pakistan. BMC infectious diseases. 2019;19:1-13. Doi.org/10.1186/s12879-019-4590-2.
William T, Jelip J, Menon J, Anderios F, Mohammad R, Awang Mohammad TA, et al. Changing epidemiology of malaria in Sabah, Malaysia: increasing incidence of Plasmodium knowlesi. Malaria journal. 2014;13(1):1-11. Doi.org/10.1186/1475-2875-13-390
Nigussie TZ, Zewotir TT, Muluneh EK. Seasonal and spatial variations of malaria transmissions in northwest Ethiopia: Evaluating climate and environmental effects using generalized additive model. Heliyon. 2023;9(4). gussie). Doi.org/10.1016/j.heliyon.2023.e15252

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