In Vitro Antimicrobial Activity of Garlic (Allium Sativum) Against Clinical Isolates of Vibrio Cholerae
Introduction: To study the in vitro antimicrobial
activity of garlic (Allium Sativum) against clinical
isolates of Vibrio Cholerae
Methods: Thirty three clinical isolates of V.
cholerae were evaluated for their susceptibility to
aqueous extract of Pakistani and Chinese garlic.
ATCC strain of P. aeruginosa (ATCC 27853) was
used as the standard control strain. The cloves were
peeled and blended (50 gm) in 90 ml of distilled
water in separate blenders. The extracts were
sterilized by gamma radiation. Aqueous extracts of
Pakistani and Chinese garlic were tested in
triplicate. Quantitative analysis of Pakistani and
Chinese garlic aqueous extracts was done. As the
extracts of both types of Pakistani and Chinese garlic
exhibited inhibitory effect against test strain of V.
cholerae, aqueous extracts were further evaluated in
agar dilution assay to determine MIC against thirty
three clinical isolates of V. cholerae and one ATCC
Results: The zones of inhibition measured by using
agar well diffusion assay were 28.12 mm and 32.14
mm for Pakistani and Chinese garlic aqueous
extracts respectively. All the isolates were inhibited
at concentration of 8.66 mg/mL by Chinese garlic and
at 10 mg/mL by Pakistani garlic aqueous extract.
Conclusion: Natural sources, like garlic extracts ,
may be an effective alternative, to resistant strains of
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